=============================================================== == == == ----------- ALS INTEREST GROUP ----------- == == ALS Digest (#51, 17 JULY 1993) == == == == To subscribe, to unsubscribe, to contribute notes, etc. == == to ALS Digest, please send e-mail to: == == bro@huey.met.fsu.edu (Bob Broedel) == == == == All interested people may "broadcast" messages to == == ALS Digest subscribers by sending to: == == als@huey.met.fsu.edu == == == =============================================================== This entire issue is about BDNF (brain-derived neurotrophic factor). (1) ===== Clinical trials of BDNF on ALS patients ========== The latest edition of the list, DRUG TRIALS FOR ALS PATIENTS (revised 06/25/93) is available from the ALS Association. It includes information about BDNF clinical trials that are to begin in August of 1993. Interested people should get a copy of this list from: ALS Association; 21021 Ventura Blvd., Suite 321; Woodland Hills, CA 91364-2206. TEL 818-340-7500, FAX 818-340-2060. (2) ===== The following was taken from the GenBank Data Base (GDB). ========== Subject: Locus: BDNF : brain-derived neurotrophic factor : 11p13 ****************************************************************** - GDB Locus Detail - GDB Id: Symbol: BDNF Type: Gene Locus Name: brain-derived neurotrophic factor Cyto Location: 11p13 Assign. Modes: Annealing of Homologous DNA-DNA or DNA-RNA Sequences; Somatic Cell Hybrids Genbank: M37762,M37763,M61175,M61178,M83377 Allele Max Locus Set Type Probe/Enzyme Het -------------------------------------------------------------------- BDNF Aa DINUC BDNF.PCR1.1/BDNF.PCR1.2 0.56 Primary Author Citation Year -------------------------------------------------------------------- Jones, KR Proc Natl Acad Sci U S A 87:8060-4 1990 Maisonpierre, + Genomics 10:558-68 1991 Ozcelik, T Genomics 10:569-75 1991 Hanson, IM Genomics 13:1331-3 1992 Proschel, M Hum Mol Genet 1:353 1992 Smith, MW Personal Communication 1993 Probe Name Type DNA Type Locus ------------------------------------------------------------------- BDNF.Clone1 Cloned Genomic BDNF pTB1428 Cloned Genomic BDNF BDNF.2 Cloned Genomic BDNF pSK-hB(C1) Cloned cDNA BDNF BDNF.PCR1.1/BDNF.+ PCR Genomic BDNF(+) bdnf-A/bdnf-Z PCR Unknown BDNF (3) ===== Here is info about some of the citations re BDNF ========== (a)----- Subject: Cit: Jones, KR : PROCEEDINGS OF THE NATIONAL ACADEMY OF S+ : 87:8060-4 : 1990 ******************************************************************** - GDB Citation Detail - GDB Id: Citation Type: Journal Authors: Jones, KR; Reichardt, LF Title: Molecular cloning of a human gene that is a member of the nerve growth factor family. Journal: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA Volume: 87 Issue: 20 Pages: 8060-4 Year: 1990 Locus Assign Symbol Mode(s) Name Location -------------------------------------------------------------------- BDNF A,S brain-derived neurotrophic fa+ 11p13 NTF3 A neurotrophin 3 12p13 Probe Name Type DNA Type Locus -------------------------------------------------------------------- BDNF.Clone1 Cloned Genomic BDNF hNT3/BR6 Cloned Genomic NTF3 (b)----- Subject: Cit: Shintani, A : BIOCHEMICAL AND BIOPHYSICAL RESEARCH COM+ : 182:325-32 : 1992 *********************************************************************** - GDB Citation Detail - GDB Id: Citation Type: Journal Authors: Shintani, A; Ono, Y; Kaisho, Y; Igarashi, K Title: Characterization of the 5'-flanking region of the human brain-derived neurotrophic factor gene. Journal: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Volume: 182 Issue: Pages: 325-32 Year: 1992 Probe Name Type DNA Type Locus ---------------------------------------------------------------------- pTB1428 Cloned Genomic BDNF (4) ===== Here are some contact people re BDNF ========== (a)----- Subject: Contact: George D. Yancopoulos : Tarrytown USA ************************************************************************ - GDB Contact Detail - Contact Name: George D. Yancopoulos Regeneron Pharmaceuticals, Inc. 777 Old Saw Mill Road City: Tarrytown State: NY Postal Code: 10591 Country: USA Phone: Fax: Email: Probe Name Type DNA Type Locus GDB Id ----------------------------------------------------------------------- BDNF.2 Cloned Genomic BDNF pSK-hB(C1) Cloned cDNA BDNF PhihN3(G1) Cloned Genomic NTF3 (b)----- Subject: Contact: Suzanne Clancy : San Diego USA ********************************************************************** - GDB Contact Detail - Contact Name: Suzanne Clancy The Salk Institute for Biological Studies Molecular Genetics Lab P.O. Box 85800 City: San Diego State: CA Postal Code: 92186-5800 Country: USA Phone: 1 (619) 453-4100 Ext. 2 Fax: 1 (619) 588-9513 Email: Probe Name Type DNA Type Locus GDB Id ------------------------------------------------------------------------------- OSM-13.5 Cloned Genomic OSM cSRL-175a5 Cloned Genomic CNTF cSRL-98H9 Cloned Genomic CNTF cSRL-131G3 Cloned Genomic CNTF bdnf-A/bdnf-Z PCR Unknown BDNF many, many others (too many to list here) (c)----- Subject: Contact: Peter Maisonpierre : Tarrytown USA ********************************************************************* - GDB Contact Detail - Contact Name: Peter Maisonpierre Regeneron Pharmaceuticals 777 Old Saw Mill River Rd. City: Tarrytown State: NY Postal Code: 10591-0707 Country: USA Phone: 1 (914) 345-7409 Fax: 1 (914) 347-5045 Email: Probe Name Type DNA Type Locus --------------------------------------------------------------------- pSK-hB(C1) Cloned cDNA BDNF PhihN3(G1) Cloned Genomic NTF3 (d)----- Subject: Contact: Asae Shintani : Osaka Japan ********************************************************************** - GDB Contact Detail - Contact Name: Asae Shintani Biology Research Laboratories Takeda Chemical Industries, Ltd. 2-17-85 Jusohonmachi Yodogawa-ku City: Osaka State: Postal Code: 532 Country: Japan Phone: 81-06-300-6868 Fax: 81-06-300-6306 Email: Probe Name Type DNA Type Locus --------------------------------------------------------------------- pTB1428 Cloned Genomic BDNF (5) ===== The following was taken from the NSF Gopher ========== Subject: Title: BDNF, NT-3, and LIF Stimulation of Cholinergic Neuron This section is from the document '/.index/ftp/awards91/awd9112/a9112913'. Title : BDNF, NT-3, and LIF Stimulation of Cholinergic Neuron Development Type : Award NSF Org : IBN Date : April 06, 1993 File : a9112913 Award Number: 9112913 Award Instr.: Continuing Grant Pgrm Manager: Steven C. McLoon IBN DIV OF INTEGRATIVE BIOLOGY & NEUROSCIENC BIO DIRECT FOR BIOLOGICAL SCIENCES Start Date : July 1, 1991 Expires : June 30, 1994 Amt to Date : $190,911 Investigator: Franz F Hefti Sponsor : University of Southern California Andrus Gerontology Center University Park Los Angeles, CA 90089 213/743-2311 NSF Program : 1190 DEVELOPMENTAL NEUROSCIENCE Fld Science : 61 Life Science Biological Fld Applictn: 0000099 Other Applications NEC Dissociated cultures of neurons obtained from various rat brain regions will be employed to study the effects of the newly discovered neurotrophic factors, brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and leukemia inhibitory factor (LIF) on cholinergic and dopaminergic biochemical differentiation. Observed effects will be compared to those of nerve growth factor (NGF). In vivo experiments in which the neurotrophic factors are injected into specific brain areas will be done to determine whether the effects observed in culture are biologically relevant. == end of ALS 51 ==